Stuck in an ice-cream tub

So, a little update on "Micro Bee", whom we rescued last weekend. Well, she is now known as "Beatrice" or "Bea" and was named by my niece Chloe, who also named Holly (our disabled bee) and Fifi (our original Koppert Queen). 

So, a quick recap: we brought Bea indoors after watching her leave the nest and walk across the lawn (only a few feet) but not succeed in flying. She is absolutely tiny - about the size of a small fly - but perfectly formed, at least visually (i.e not missing limbs, all the right colours etc.). However, her wings are slightly bent and we think this is the reason she doesn't seem to fly. 

I honestly can't describe how small she is - here is a picture of her in her tub to give you an idea:

little Bea - trying to get her to fly

So, we've followed the same routine we did with Holly and BLB  - both of whom were disabled and couldn't fly - and kept her indoors in a small ice cream tub, with some materials to explore and a supply of known/safe honey water. 

We tend to her regularly but try to allow her to experience natural daylight hours. Every day we take her out (as above) and give her the opportunity to try and fly, but has not tried to do so. There's no real excuse for her not doing so at the moment, given the unnaturally warm temperatures we are experiencing (about 29 degrees C), so she could easily get up to temperature. 

It's not ideal keeping her indoors and we are debating whether to try and return her to the nest. The problem is, if she wanders out again without our supervision, she could easily get lost on the lawn and run out of energy and/or quickly become prey. Quite simply, she is just not equipped for life outside the nest. 

I have the following concerns keeping her indoors:

• could it be affecting her 'mood' - there is evidence from honey bees that their disposition can be affected by negative events, to the extent that they show signs of "depression" (altered behaviour states and less persistence, i.e. giving up more quickly). I would hate it if this was happening.
• Bumblebees are basically social creatures; their behaviour a jigsaw piece in the bigger colony system. So, by being away from the colony, both the isolated bee and the colony could be affected. Quite simply, Bea cannot fulfil her role or instincts if separated from her siblings. 
• Does it change her behaviour patterns?

Actually, we have pretty strong evidence that behaviour patterns are changed, not just from Bea but from holly  et al. we kept indoors before her. The first 24 hours is spent in a very active and exploratory phase, as you would expect. when given the chance to explore (e.g. taking the lid off outside) they try to escape (or at least explore beyond the boundaries of their confines).

Then next few days this really calms down, essentially coming to a halt and there appears to be a sense of resigned stillness. There is little activity, often they hide under the moss and seem to spend a lot of time "sleeping". This is very much like a "low mood" creeping in, having learnt their surroudings and discovered they are alone and trapped. 

Over the next few days they then appear to become "masters" (or mistresses I suppose!) of their environment. A strong sense of territory and defence appears to develop. At this stage, opening the tub is greeted with warning signs, such as raised leg. As the days go by this becomes even stronger with different levels of warning - the final stage being lying on the back with sting pointed at you. What's more, the response at this stage is instantaneous; without a moment's delay the bee throws itself onto its back and warns with its sting. It becomes very protective of its food supply, which leads to more warnings as this is topped up with a syringe.  When taken outside and given the opportunity to explore/fly, there is no apparent interest in doing so. Indeed, the behaviour at this stage is now attending to and defending the nest/environment with no requirement for exploration beyond those boundaries. 

This is the stage that Bea is at, so trying to get her to fly now seems completely fruitless and adds to the dilemma of whether she is now so conditioned to her tub environment that trying to return her to the nest is counter-productive.

We've only had a Bea for a week so that's as far as her behaviour has developed; but, of course, we had Holly for much longer and saw these patterns develop further, such that warning signs subsided and indeed there was a complete acceptance of intervention with the food supply.

Indeed, eventually Holly learnt that the syringe meant new food and would come to get it even before we had chance to administer it. She was also completely happy drinking while we were topping up. Quite remarkable really. She also went into a much more intense "nest fixing" mode, constantly working at the nest. Rarely she would come out of the nest to check the immediate surroundings then return. Interestingly (and perhaps not surprisingly) she was stimulated by other bees and was far more active when they were in her environment. 

Anyway, that's a summary of some of the behaviour - I intend to produce a more detailed write up in due course. 

Back to the nest?

If we do add Bea back to the nest, there are two key risks. The first is she simply just starts to explore again to discover her new environment and ends up tired and lost outside of the nest. The second is that she tries to perform like her tiny siblings, one at least of which has been collecting pollen. And befalls the same fate. So either way the outcome is the same. 

tiny bee working to collect pollenPurely for interest, above is a picture of one of her equally tiny colleagues on the way out to collect pollen. This wonderful miniature bundle of bumbleness has been busy foraging and managing to find a source of lovely bright orangey pollen - possibly late flowerign lavender (of which we have some). She comes back with her legs buckling under the 'vast' quanity she has collected. 

She's actually one of the most active bees in the nest and she (or an identical looking sister) has the role of some patrolling and nest checking, especially at night. When we opened the nest for a quick look last night, there she was scooting round the perimeter of the plastic box ensuring everything was safe; with a little warning fizz on occasions just to keep us in check. 

She may be small, but she's taken on a big role. 

Pattern Matching

So, we finally came home and were able to examine the mayhem created by our two disabled bees in their plastic tub. Actually when we saw it in person, as opposed to the limited remote camera view, it probably wasn't quite as bad as it seemed. That's not to say they hadn't moved loads around, but it seemed to be slightly more organised that we imagined. 

Organised? That's not a word we've been applying to Bumblebees! 

Here's (more or less) how we left the box:

original layout - the moss is all along one side of the box

The easiest way to describe what they'd done was move moss away from the edges and corners towards the centre. What's not obvious is the cause and effect - whether the movement of the moss is merely a result of the endless patrolling of the perimeter of the box (e.g. a security or escape motivation), or whether the intention is to collect the moss centrally by wearing a path round the edges (i.e. keep the edges clear with a fixing/nest building motivation).

The box as we found it after the bees had it for 48 hours

We surmise there is a possiblity it is the latter - and that the bees are programmed to keep their nest material away from the edges of the nest in order to keep it dry. Certainly we have read this as advice when preparing a nest box, which would mimic what would happen underground. 

Is there method in the madness?

So, although much of their behaviour seems quite erratic, every once in a while it does seem to follow certain patterns. I'd classify these as:

• Traversing the perimeter, although often this involves traversing part of it, then returning to a designated "safety spot", more centrally located
• exploring outward in a "hub" fashion from said "safety spot" - i.e. heading out to explore a portion of the box, then returning (or partially returning) before tackling the next segment
• circling the same area, usually in fairly tight circles. When this behaviour is done on in amongst the moss or nest material, it moves it and creates the tunnels and "whorls" that (for example) we've seen the Queens create in bedding. It also creates the space in the corners. 
• Sticking the face in the moss and the bottom out/in the air - this seems to happen when one bee wants to ignore the other (e.g. appears to want to rest without disturbance) but there are also other times when they are resting. In a way it seems quite a vulnerable position, not being able to see any threat, although the sting is foremost. Also, the camouflage of the bands works in their favour and perhaps they just need a bit of dark once in a while. 
• There are times when they seem to take turns with role swapping - for example, one bee will be taking a rest, buried in the moss while the other is scurrying about circling the box and the moss. Then after 15 or 20 minutes it will approach the rest bee and prod it and shove it and basically wake it up! Then it will tend to take over the scurrying and "nest fixing" behaviour. Although Holly (the smallest) was the main protagonist ("agitator") to begin with, we've subsequently seen both bees demonstrating this behaviour. 

 I was keen to do some experiments, for example to see if they would exhibit these behaviours with different materials, with different box orientations and layouts. However, it's difficult to devote the time to study this with sufficient scientific accuracy, so we can really only report anecdotal observations.

Moreover, events have taken over us, in that we've now moved the bees to a larger habitat - more on that on a follow up blog :-)

Here's a little bit of exploratory behaviour...

I spy with my little eye

For the last few days I have been looking at the overnight DVR footage each morning to see if there are any interesting. Here's a few things we have noticed:

1. Starting the day

The first bumblebees start to emerge from the nest around the time the sun starts to shine directly on it. We can tell this because when the sun shines directly into the nest (it faces East) it triggers the internal camera to switch off the infra-red and we can see this happen on our video recordings. It's around this time we then start seeing the first bees heading off to fields. It's also easy to see them exit the nest as when they enter the entrance tube, they block the sunlight momentarily and the camera switches back to infra-red. This appears as a kind of "flashing" effect on the video and gives us a second or two notice that a bee is about to emerge! 

This actually ties in with behaviour we saw with our queens when they used to emerge from the front nestbox at around 10am. Although this box faced North, it was around this time that the sun would shine off our window and bounce directly onto the nestbox entrance. This currently suggests to us that the bees' outdoor exploratory behaviour is triggered by the levels of sunlight entering the nest. It probably stands to reason, but the beauty of observing in the 'wild' rather than in a lab is we can see this correlation more directly. 

2. The first trip of the day is the "paper run"

At the time of writing (mid May) our bees are first emerging from the colony at around 7am in the morning. As it happens, over an hour before that, we saw a Common Carder bee on the lavender right next to our nest. We don't know if being an ealier riser is symptomatic of their type, or just down to the location of their nest, light levels and so on. 

Assuming, however, that we don't have any bees staying out overnight from the nest (a possiblity if they get caught out in bad weather), then we can pretty much time how long these early bees spend out when they first leave. This morning we were able to count about 4 out and 4 back in between about 7.30 and 8am.

On average they were spending about 15 minutes away from the nest. They are not coming back laden with pollen at this time, perhaps just partaking of a light organic breakfast!

3. Young are hatching in increasing numbers

We cannot see the hatching area inside the nestbox. The main reason is the camera is not pointing at it! - but that's because we were unable to find it. The true layout of the box will not be revealed till the end of the season when we can deconstruct it - but it seems that most of the action is happening below a rather sturdy (possibly supported) layer of nesting material which we cannot get access to.

However, we know that young are hatching because we are seeing them leave the nest. And we know it's the young leaving the nest because they perform a special "memorisation" and "landmarking" routine when they leave the nest for the first time. 

This is quite a fascinating and recognisable routine, so I've put some videos together below - the first one shows their behaviour close the entrance as they memorise it. The second one (about 30 seconds in) shows the wider circling behaviour as they map out the landmarks in the nearby area:

Between 7am and Midday we counted about half-a-dozen new bees today - a record so far.

We also observed one or two bees actually performing this type of behaviour on the way IN to the nest (you can see one at the end of this video). This has us slightly baffled - at the moment our best hypothesis is that perhaps these are bees on the first time back to the nest and they are re-affirming their memory of the nest, double-checking they are not heading into the wrong territory. Pure speculation on our part. 

There's not much time to sleep

 We can use the motion detection feature on the DVR to monitor how much activity occurs in the nestbox during the night. We were very interested to see if the bees ever really went to sleep or carried on being busy throughout the night. What we've observed is that there is less activity during the wee small hours of the night but they never really stop completely. The quietest time is between 2am and 3am - during that time, for example, there might be one or two bees during the whole hour that come into view of the camera. Outside of those hours the rate is higher. 

At some point I'm hoping to count these up and produce some firmer stats (unfortunately this is a bit of a manual task). The intention is also to correlate the daytime activity with weather data also. 

It should be borne in mind that our camera is not in the heart of the nest, but pointed over (we think) the sugar-water wick used for drinking/feeding. So, the night-time activity we see is likely to represent feeding behaviour rather than whatever-it-is might be happening deep inside the nest. One of the behaviours we also see at this position appears to be night-time perimeter patrol. These are bees that traverse the edge of the box, sometimes nudging others out of the way, but don't stop to feed or tug on the nest material. 

The idea of a security patrol is not entirely ludicrous. We have already observed on several occasions, after disrupting the nest, a lone bee coming to inspect the damage and survey the scene. Mpst revcently, when we inserted the camera into the nestbox and re-opened the hive entrance, a bee came into that entrance and sat there as if on "lookout". It didn't come out onto the box to inspect it, but just sat in the entrance, blocking it and watching us. It was a bit uncanny really!

Anthropomorphism

Of course, we don't have full scientific explanations for all the activities we see, so it's easy to anthropomorphise the bee behaviours with Human explanations. On the other hand, bees are social animals, so it makes some sense that there could be roles that aid the stability of the colony.